枇杷离体胚萌芽与丛生芽诱导的研究In Vitro Germination and Multiple Shoot Induction of Immature Embryos in Loquat
孔素萍,王永清
KONG Su-ping WANG Yong-qing (College of Forestry and Horticulture;Sichuan Agricultural University;
摘要(Abstract)：
采用枇杷胚为外植体 ,以 1/ 2MS为基本培养基 ,采用正交试验研究了不同浓度的激素对胚萌芽的影响 ,筛选出了最佳萌芽培养基 1/ 2MS +6 BA2 .0mg/L +LAA0 .5mg/L +NAA0 .0mg/L。同时也比较了时间对萌芽的影响。丛生芽诱导与增殖以MS +6 BA2 .0mg/L +NAA0 .2mg/L为较好培养基 ,并且高浓度的 6 BA不利于无根苗健康生长 ,而NAA对此无影响。
The immature embryos of loquat were used for explants in the study.The effects of plant growth regulators on in vitro germination of loquat embryos were investigated using orthogonal design method.The best embryo germination was obtained on 1/2 MS+6-BA 2.0 mg/l+IAA 0.5 mg/l+NAA 0.0 mg/l.The effects of sampling time were also studied.The inducation and multiplication of tufty buds were nice on MS+6-BA 1.0 mg/l+NAA 0.2 mg/l-And 6-BA at higher concentration was not suitable for the growth of the plantlets without roots.But NAA did not have the effect.
关键词(KeyWords)：
枇杷;幼胚;离体培养
loquat,immature embryo,In vitro culture
基金项目(Foundation): 教育部高校骨干教师项目;; 四川科技厅应用基础研究项目提供资助
作者(Authors):
孔素萍,王永清
KONG Su-ping WANG Yong-qing (College of Forestry and Horticulture;Sichuan Agricultural University;
DOI: 10.16779/j.cnki.1003-5508.2002.01.012
参考文献(References)：
- [1] 杨永青,陈光禄,唐道一.枇杷茎尖培养与增殖的研究[J].园艺学报.1983,10(2):79～85.
- [2] 李春瑶,吴国团,唐连英,等.枇杷成年茎段离体培养及植株再生[J].广西师范大学学报.1995,13(3)77～80.
- [3] 万志刚,宋卫平,顾福根,等.良种白沙枇杷“冠玉”的组织培养[J].植物生理学通讯.2000,36(4):338～339.
- [4] 庄馥萃,潘维雄,吴金珠.枇杷未成熟胚的培养[J].亚热带植物通讯.1980,(2):3～7.
- [5] 何伟其.枇杷幼胚及茎顶培养诱导细胞发生的研究[J].中国园艺.1983,29(4):322.
- [6] 林顺权,凌经天,仁藤伸昌.枇杷胚培养及其原生质体的分离与培养[J].(日本)园艺学杂志.1989,58(2):48～49.
- [7] LaneWDAdventitiousshootsfromcotyledonsofimmaturecherryapricotembryos.Can.J .PlantSci.1986,66(4):953～959.
- [8] 陈香波,曹孜义.离体葡萄未成熟胚成苗途径研究[J].果树科学.2000,17(4):261～264.