杉木优良无性系组培繁育技术研究A study of Culture in Vitro and Propagation of Superior Clones of Cunninghamia lanceolata
刘海鹰,万雪琴,刘均利,杨晓蓉
LIU Hai-ying,WAN Xue-qin,LIU Jun-li,YANG Xiao-rong
摘要(Abstract)：
以杉木(Cunninghamia lanceolata Hook.)优良无性系3-3的基部萌条为外植体进行了组培快繁技术研究。试验结果表明:截干的最佳时间是在3月中旬,截干高度为30 cm,平均萌芽数达到47.2条;外植体最佳消毒方案是70%酒精浸泡15 s,后无菌水洗涤2次,再用0.1%升汞浸泡消毒6 min,无菌水洗涤6次;初代诱导最适基本培养基是MS培养基;增殖诱导最适培养基为MS+BA(0.5 mg·L~(-1)~0.7 mg·L~(-1))+IBA(0 mg·L~(-1)~0.2 mg·L~(-1));生根的最适培养基为1/2MS~1/4MS+0.5 mg·L~(-1)~1.0 mg·L~(-1)IBA+0.1 mg·L~(-1)NAA;杉木组培苗移栽的最适基质是泥炭土∶黄心土∶蛭石=4∶2∶1的混合土。
Culture in Vitro and propagation were carried out by using basal sprouts of superior clones 3-3Cunninghamia lanceolata as explants. The result showed that the optimal cutting trunk height was 30 cm in March and the average number of bud was 47. 2; Best explants disinfection solution was to immerse15 s in 70% alcohol and use sterile water to wash twice and then to soak 6 min in 0. 1% mercuric chloride and wash 6 times with sterile water; MS culture medium was the optimal basic culture in primary culture;Subculture medium was MS + BA( 0. 5 mg·L~(-1)~ 0. 7 mg·L~(-1)) + IBA( 0 mg·L~(-1)~ 0. 2 mg·L~(-1));Inducing-root medium was 1 /2MS ~ 1 /4MS + 0. 5 mg ·L~(-1)~ 1. 0 mg · L~(-1)IBA + 0. 1 mg · L~(-1)NAA;The most suitable soil matrix proportion of transplanting was peat soil∶ yellow subsoil∶ vermiculite = 4 ∶ 2 ∶1.
关键词(KeyWords)：
杉木;优良单株;促萌;组织培养
Cunninghamia lanceolata Hook;Superior clones;Sprout promoting;Tissue culture
基金项目(Foundation): 四川省“十二五”育种攻关项目“突破性经济林(竹)新品种选育”(2011NZ0098-10);; 四川省公益性科研院所基本科研项目“杉木优良无性系规模化快繁技术研究”(JB2014-10);四川省公益性科研院所基本科研项目“杉木优良单株选择及组培快繁技术研究”(JB2015-10);四川省公益性科研院所基本科研项目“杉木组培快繁技术体系优化研究”(JB2016-12)
作者(Author):
刘海鹰,万雪琴,刘均利,杨晓蓉
LIU Hai-ying,WAN Xue-qin,LIU Jun-li,YANG Xiao-rong
DOI: 10.16779/j.cnki.1003-5508.2016.05.001
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