朱天辉,李姝江,向潇潇,谯天敏
ZHU Tian-hui LI Shu-jiang XIANG Xiao-xiao QIAO Tian-min(College of Forestry,Sichuan Agricultural University,Ya'an 625014,China)

• 1:四川农业大学林学院

摘要(Abstract)：

采用单因素试验和正交试验对地衣芽孢杆菌(Bacillus lincheniformis)YB15发酵培养基的碳源、氮源、无机盐进行研究,得出该菌的最适培养基组分为葡萄糖5 g,酵母浸膏7.5 g,蛋白胨7.5 g,硫酸铵5.0 g。在此基础上运用正交试验对地衣芽孢杆菌YB15发酵的最适条件进行研究,得到的最适培养条件为温度25℃,pH 6.5,瓶装量100 mL,接种量4 mL;地衣芽孢杆菌生长曲线的结果表明12 h～18 h时为菌体收集的最佳时间。
In this paper,by adopting the single-factor and orthogonal experiments the researches were conducted on the fermentation medium of Bacillus licheniformis YB15,and the results showed that its optimum components were glucose 5.0 g,yeast extract 7.5 g,peptone 7.5 g and ammonium sulphate 5.0 g.On this basis,its optimum fermentation conditions were also studied,which were temperature at 25 ℃,pH 6.5,culture broth quantity 100 mL·300 mL-1 flask and inoculation quantity of 4 mL.The result of the growth curve of Bacillus licheniformis showed that 12~18 hours were the best time to collect it.

关键词(KeyWords)： 地衣芽孢杆菌;发酵培养基;组分优化;生长曲线
Bacillus licheniformis,Fermentation medium,Optimal cultivated condition,Growth curve

Abstract:

Keywords:

基金项目(Foundation): 国家自然科技资源共享平台(2005DKA21207-13)

作者(Author): 朱天辉,李姝江,向潇潇,谯天敏
ZHU Tian-hui LI Shu-jiang XIANG Xiao-xiao QIAO Tian-min(College of Forestry,Sichuan Agricultural University,Ya'an 625014,China)

DOI: 10.16779/j.cnki.1003-5508.2013.01.001

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