李诗娟,宋祖文,王丽,黄仲华,盛玉珍,金开铭,张玲,徐磊
LI Shi-juan,SONG Zu-wen,WANG Li,HUANG Zhong-hua,SHENG Yu-zhen,JIN Kai-ming,ZHANG Ling,XU Lei

• 1:四川省林业科学院
• 2:四川林业宣传中心
• 3:喜德县林业局

摘要(Abstract)：

为获得高效的溶磷菌,用于菌肥生产。通过Pikovskava无机解磷培养基从核桃土壤里筛选出溶磷菌,通过液体发酵试验确定其溶磷活力,利用分子生物学方法对较高活力的溶磷菌进行分子鉴定;通过单因素试验,比较该溶磷菌与两种菌肥溶磷菌在不同发酵液配方及发酵条件下的发酵活力。分离得到的高效溶磷菌为芽孢菌属,适宜初始p H值范围为5~9,适宜温度范围为16℃~40℃,适宜氮源有硫酸铵、氯化铵和草酸铵、蛋白胨,适宜碳源有草酸铵、蔗糖、甘油;高浓度Fe~(3+)、Mn~(2+)对其活力有一定的促进作用,而Cu~(2+)、Ni~(2+)、Zn~(2+)会抑制其解磷。在不同发酵液配方及发酵条件下,该溶磷菌溶磷能力优于菌肥2溶磷菌;在不同发酵条件下,该溶磷菌溶磷能力稳定性优于菌肥1溶磷菌。该溶磷菌添加到菌肥中有一定的市场潜力。
It aimed to gain highly efficient phosphate-solubilizing microorganisms. Phosphate-solubilizing bacteria were isolated in advantage of Pikovskava phosphate dissolving medium. Liquid fermentation experiments showed the phosphate-solubilizing activity,molecular biological methods identified the microbial functional community with higher activity. By single factor test,the vitality of different strains were compared under different formulas and the fermentation conditions of culture medium. Bacillus sp. was the phosphate-solubilizing bacterium with higher efficiency. The optimum medium was as follows:suitable initial p H of 5 ~ 9,optimum temperature range of 16 ℃ ~ 40 ℃,ammonium sulphate,ammonium chloride and ammonium oxalate,peptone for suitable nitrogen source,ammonium oxalate,sucrose,glycerol for suitable carbon source. High concentration of Fe~(3+)and Mn~(2+)had a certain role in promoting its vitality,but Cu~(2+),Ni~(2+)and Zn~(2+)would hold the phosphate solubilizing. In different formulations and fermentation conditions,Its phosphate solubilizing ability was better than that of microbial fertilizer 2. In different fer-mentation conditions,the stability of phosphate solubilizing ability was better than that of microbial fertilizer 1. It would give a certain market potential for the phosphate-solubilizing bacteria added to the fertilizer.

关键词(KeyWords)： 溶磷菌;溶磷能力;优化
Phosphate-solubilizing bacteria;Phosphate-solubilizing ability;Optimization

Abstract:

Keywords:

基金项目(Foundation): 四川林业厅项目

作者(Author): 李诗娟,宋祖文,王丽,黄仲华,盛玉珍,金开铭,张玲,徐磊
LI Shi-juan,SONG Zu-wen,WANG Li,HUANG Zhong-hua,SHENG Yu-zhen,JIN Kai-ming,ZHANG Ling,XU Lei

DOI: 10.16779/j.cnki.1003-5508.2018.04.005

参考文献(References)：

• [1]姜瑞波,张晓霞.微生物肥料的种类及其应用[J].磷肥与复肥,2002,17(3):10~11.
• [2]沈其荣,谭金芳,钱晓晴.土壤肥料科学通论[M].北京:高等教育出版社,209~213.
• [3]赵小蓉,林启美,孙焱鑫,等.玉米根际与非根际解磷细菌的分布特点[J].生态学杂志,200l,20(6):62~64.
• [4]Vig A C,De V G.Phosphoms adsorption characteristics of some acid and alkaline soils,Joumal of the Indian Society of Soil Science,1984,32(2):235~239.
• [5]Bagyaraj D J,Krishnaraj P U,Khanuja S P S.Mineral phosphate solubilization:Agronomic implications,mechanism and molecular genetics,Proceedings of the Indian National Science Academy,2000,66(2):69~82.
• [6]林启美,王华,赵小容,等.一些细菌和真菌的解磷能力及其机理初探.2001,28(2):27.
• [7]王舒,张林平,张扬,等.红壤区油茶根际解磷细菌的筛选、鉴定及其解磷能力[J].林业科学研究,2015,28(3):409~416.
• [8]黄达明,李倩,管国强,等.一株解磷细菌的筛选、鉴定及其溶磷培养条件的优化[J].生物技术通报,2015,31(2):173~178.
• [9]刘云华,吴毅歆,杨绍聪,等.洋葱伯克霍尔德溶磷菌的筛选和溶磷培养条件优化[J].华南农业大学学报,2015,36(3):78~82.
• [10]邵锴,邱业先,徐婧.高效溶磷菌的筛选、鉴定及其溶磷特性[J].江苏农业科学,2017,45(8):253~257.
• [11]王誉瑶,韦中,徐阳春,等.溶磷菌株组合的溶磷效应及对玉米生长的影响[J].植物营养与肥料学报,2017,23(1):262~268.