SSR标记技术在杨属植物研究中的应用The Application of the SSR Marker to Research on Populus L.
殷继艳,张建国
YIN Ji-yan1 ZHANG Jian-guo2(1.The Services Command College of Chinese People's Armed Police Force,Beijing 102202
摘要(Abstract):
SSR标记具共显性和多态性等特点,是一种很有价值的分子标记,微卫星在林木基因组中广泛存在。本文综合国内外SSR标记在杨属植物研究中的应用,并探讨了该技术存在的问题及发展趋势。
SSR markers are valuable as genetic markers because they are co-dominant and detect high levels of allelic diversity and the most abundant microsatellite motifs reported in forest.The application of the SSR markers to the research on Populus L.and the existing problems and prospect of this technology are also discussed in this paper.
关键词(KeyWords):
杨属植物;SSR标记;分子标记
Populus L.,SSR markers,Genetic markers
基金项目(Foundation):
作者(Author):
殷继艳,张建国
YIN Ji-yan1 ZHANG Jian-guo2(1.The Services Command College of Chinese People's Armed Police Force,Beijing 102202
DOI: 10.16779/j.cnki.1003-5508.2009.02.006
参考文献(References):
- [1]徐立安等.用SSR研究栲树群体遗传结构[J].植物学报,2001,43(4):409~412.
- [2]M.H.Rahman.O.P.Rajora.Microsatellite DNA somaclonal varia-tion in micropropagated trembling aspen(Populus tremuloides).Plant Cell Rep,2001,20:531~536.
- [3]M.J.M.Smulders,J.Vander Schoot.Trinucleotide repeat microsat-ellite markers for black poplar(Populus nigra L.).Molecular E-cology Notes,2001,1:188~190.
- [4]Eric imbert.Dispersal and gene flow ofPopulus nigra(Salicace-ae)along a dynamic river system.Journal of Ecology,2003,91:447~456.
- [5]T.Fossati,F.Grassi.Molecular analysis of natural populations ofPopulus nigra L.intermingled with cultivated hybrids.Molecular E-cology,2003,12:2033~2043.
- [6]T.Fossati.Development of molecular markers to assess the level ofintrogression ofPopulus tremulainto P.alba natural populations.Plant Breeding,2004,123:382~385.
- [7]汪小全.植物分子系统学近五年的研究进展概况[J].植物分类学报,1997,35(5):465~480.
- [8]李云海,肖含,等.用微卫星DNA标记检测中国主要杂交水稻亲本的遗传变异[J].植物学报,1999,41(10):1061~1066.
- [9]李新军,黄敏仁,潘惠新.林木基因组中的微卫星(SSR)及其应用[J].南京林业大学学报,1999,23(5):64~69.
- [10]S.Dayanandan,Kamaljit S.Bawa,and Rick Kesseli.Conservationof Microsatellites among Tropical Trees(EGUMINOSAE).Amer-ican Journal of Botany,1997,84(12):1658~1663.
- [11]殷继艳.新疆额尔齐斯河流域杨属系统发育和种间关系研究.博士论文,2006.
- [12]Dayanandan,S.,O.P.RAjora&K.S.Bawa.Isolation and charac-terization of microsatellites in trembling aspen(Populus tremu-loides).Theoretical and Applied Genetics,1998,96:950~956
- [13]Schoot,J.V.D.,M.PospiSkova.,B.Vosman&M.J.M.Smulde-rs.Development and characterization of mocrosatellite markers inblack poplar(Populus nigra L.).Theoretical and Applied Genet-ics,2000,101:317~322.
- [14]Rahman,M.H.,S.Dayanandan&O.P.Rajora.MicrosatelliteDNA markers inPopulus tremuloides.Genome,2000,43:29l3~29.
- [15]Rajora.O.P.&H..R.Muhammad.Microsatellite DNAand RAPDfingerprinting,identification and genetic relationships 0f hybridpoplar(Populus×canadensis)cultivars.Theoretical and AppliedGenetics,2003,106:470~477.
- [16]Christopher T.Cole.Allelic and population variation of microsatel-lite loci in aspen(Populus tremuloides).New Phytologist,2005,167:155~164.
- [17]Squirrell J,Hollingsworth PM,Woodhead M,Russell J,Lowe AJ,Gibb M,Powell W.Howmuch effort is required to isolate nuclearmicrosatellites from plants.Molecular Ecology,2003,12:1339~1348.
- [18]Luikart G,Sherwin WB,Steele BM,Allendorf FW.Usefulness ofmolecular markers for detecting population bottlenecks via monito-ring genetic change.Molecular Ecology,1998,7:963~974.
- [19]Beaumont MA.Detecting population expansion and decline usingmicrosatellites.Genetics,1999,153:2013~2029.
- [20]Garza JC,Williamson EG.Detection of reduction in populationsize using data from microsatellite loci.Molecular Ecology,2001,10:305~318.
- [21]Leberg PL.Estimating allelic richness:effects of sample size andbottle necks.Molecular Ecology,2002,11:2445~2449.
- [22]Hamrick JL,Godt MJW,Sherman-Broyles SL.Factors influen-cing levels of genetic diversity in woody plant species.New For-ests,1992,6:95~124.
- [23]Dayanandan,S.,O.P.RAjora&K.S.Bawa.Isolation and charac-terization of microsatellites in trembling aspen(Populus tremu-loides).Theoretical and Applied Genetics,1998,96:950~956.
- [24]Wyman J,Bruneau A,Tremblay M-F.Microsatellite analysis ofgenetic diversity in four populations ofPopulus tremuloidesinQuebec.Canadian Journal of Botany.2003,81:360~367.
- [25]李文英.蒙古栎天然群体遗传多样性研究[D].博士论文,2003.
- [26]Rajora,OP,and L.Zsuffa.Allozyme divergence and evolutionaryrelationships amongPopulus deltoides,P.nigra,andP.maximow-iczii.Genome,1990,33:44~49.
- [27]D Ovidio,R.Nucleotide sequence of a 5.8S rDNAgene and of theinternal transcribed spacers fromPopulos deltoids.Plant MolecularBiology,1992,19:1069~1072.
- [28]Rajora,O.P.&B.P.Dancik.Chloroplast DNA variation in Popu-lus.III.Novel chloroplast DNA variants in naturalPopulus×canadensishybrids.Theoretical and Applied Genetics,1995c,90:331~334.
- [29]Rajora.O.P.&B.P.Dancik.Allozyme variation and inheritancein leaves ofPopulus deltoides,P.nigra,P.maximowiczii,andP.×canadensisin comparision to those in root tips.Silvae Genetiea,1992a,41:289~292.
- [30]Dickmann,D.I.An overview of the genus Populus.In:D.I.Dickmann,J.G.Isebrands,J.E.Eckenwalder,and J.Richardson(eds),Poplar Culture in North America,1-42.NRC ResearchPress,Ottawa,ON,Canada.2001.
- [31]Krembs O.Die Graupappel in den Donau-Auen.AllgemeineForstzeitschrift,1956,11:345~347.
- [32]Adler W,Oswald K,Fischer R.Exkursions flora von Oesterreich.Verlag Eugen Ulmer,Stuttgart und Wien.1994.
- [33]Lazowski W.Auen in Oesterreich-vegetation,landschaft and na-turschutz.In:Monographien Band 81.Federal Environment Agen-cy-Vienna,Austria,1997.
- [34]C.Lexer.Barrier to gene flow between two ecologically divergentPopulus species,P.alba(white poplar)andP.tremula(Europe-an aspen):the role of ecology and life history in gene introgres-sion.Molecular Ecology,2005,14:1045-1057.
- [35]Rahman,M.H.&O.P.Rajora.Microsatellite DNA fingerprinting,diferentiation,and genetic relationships of clones,cultivars,andvarieties of six poplar species from three sections of the genusPopulus.Genome,2002,45:1083~1094.
- [36]Leena I.Suvanto.Clone identification and clonal structure of theEuropean aspen(Populu tremula).Molecular Ecology,2005,14:2851~2860.